MELK Antibody #2274
Filter:
- WB
- IP
Supporting Data
REACTIVITY | H |
SENSITIVITY | Endogenous |
MW (kDa) | 74 |
SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
MELK antibody detects endogenous levels of total MELK protein.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids near the carboxy-terminus of human MELK. Antibodies are purified by protein A and peptide affinity chromatography.
Background
MELK (Maternal Embryonic Leucine zipper Kinase, MPK38, KIAA0175) is a member of the Snf1/AMPK related kinase family. It is implicated in stem cell renewal, cell cycle progression and pre-m-RNA splicing (1-3). MELK is also a marker for self-renewing multipotent neural progenators, and may function in embryonic and postnatal forebrain development (4). While other members of this kinase family are activated by LKB1 and CAMKII mediated phosphorylation of the T-loop, MELK is not (5-7). Regulation of activation appears complex since MELK overexpressed in mammalian cells is inactive (7). Some evidence suggests that activation occurs through autophosphorylation of Thr167 and Ser171, although a number of additional autophosphorylation sites have been suggested (8). Recently, phosphorylations of Thr449, Thr451 and Thr481 have been specifically detected during mitosis, and are thought to occur via MPF and MAPK pathways (9). MELK has broad substrate specificity in vitro: substrates include ZPR9 (10), NIPP1 (3) and cdc25B (2), although the significance of MELK mediated phosphorylation of these proteins is unclear.
Finally, recent studies on human tumor samples and cell lines suggest that MELK expression is frequently elevated in cancer relative to normal tissues (11). MELK may provide a growth advantage for neoplastic cells, and may be a potential target for anti-cancer therapies.
Finally, recent studies on human tumor samples and cell lines suggest that MELK expression is frequently elevated in cancer relative to normal tissues (11). MELK may provide a growth advantage for neoplastic cells, and may be a potential target for anti-cancer therapies.
- Heyer, B.S. et al. (1999) Dev. Dyn. 215, 344-51.
- Davezac, N. et al. (2002) Oncogene 21, 7630-41.
- Vulsteke, V. et al. (2004) J. Biol. Chem. 279, 8642-7.
- Nakano, I. et al. (2005) J. Cell Biol. 170, 413-27.
- Tassan, J.P. and Le Goff, X. (2004) Biol. Cell 96, 193-9.
- Woods, A. et al. (2003) Curr. Biol. 13, 2004-8.
- Lizcano, J.M. et al. (2004) EMBO J. 23, 833-43.
- Beullens, M. et al. (2005) J. Biol. Chem. 280, 40003-11.
- Badouel, C. et al. (2006) Cell Cycle. 5, 883-889.
- Seong, H.A. et al. (2002) Biochem. J. 361, 597-604.
- Gray, D. et al. (2005) Cancer Res. 65, 9751-61.
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意,否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件,除非书 CST 的合法授书代表以书面形式书独接受, 否书均被拒书,并且无效。
专品专有“专供研究使用”的专专或专似的专专声明, 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的, 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专,且专用于研专用途。将专品用于专断、专防或治专目的, 或专专售(专独或作专专成)或其他商专目的而专专专品,均需要 CST 的专独专可。客专:(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品,或使用专品制造任何商专专品,(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专,或使用专品开专任何与 CST 的专品或服专专争的专品或服专, (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专,(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品, (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our
Trademark Information page.