R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
MEKK2 (E6C2G) Rabbit mAb #54488
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 78 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
MEKK2 (E6C2G) Rabbit mAb recognizes endogenous levels of total MEKK2 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His244 of human MEKK2 protein.
Background
Mitogen-activated protein kinase kinase kinase 2 (MEKK2/MAP3K2) belongs to the MAP3K family of Ser/Thr kinases. Research studies have demonstrated that MEKK2 plays a pivotal role in transducing mitogenic signals emanating from EGFR and FGF2R to JNK and ERK5 signaling cascades (1,2). Post-translationally, MEKK2 is regulated through multiple mechanisms, including dimerization (3,4), ubiquitination (5,6), phosphorylation (7), and methylation (8). Research studies implicate dysregulation of MEKK2 signaling in breast carcinoma (9), colorectal carcinoma (10), and pancreatic ductal adenocarcinoma (8).
- Sun, W. et al. (2003) Mol Cell Biol 23, 2298-308.
- Kesavan, K. et al. (2004) J Cell Physiol 199, 140-8.
- Cheng, J. et al. (2005) Mol Cell Biol 25, 5955-64.
- Cheng, J. et al. (2005) J Biol Chem 280, 13477-82.
- Takeda, A.N. et al. (2014) EMBO J 33, 1784-801.
- Yamashita, M. et al. (2005) Cell 121, 101-13.
- Zhang, D. et al. (2006) EMBO J 25, 97-107.
- Mazur, P.K. et al. (2014) Nature 510, 283-7.
- Mirza, A.A. et al. (2014) Biochim Biophys Acta 1843, 945-54.
- Jiang, L. et al. (2013) Oncol Lett 6, 1333-37.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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