MEKK2 Antibody #19607
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 78 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
MEKK2 Antibody recognizes endogenous levels of total MEKK2 protein. This antibody does not cross-react with MEKK3 protein.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly292 of human MEKK2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Mitogen-activated protein kinase kinase kinase 2 (MEKK2/MAP3K2) belongs to the MAP3K family of Ser/Thr kinases. Research studies have demonstrated that MEKK2 plays a pivotal role in transducing mitogenic signals emanating from EGFR and FGF2R to JNK and ERK5 signaling cascades (1,2). Post-translationally, MEKK2 is regulated through multiple mechanisms, including dimerization (3,4), ubiquitination (5,6), phosphorylation (7), and methylation (8). Research studies implicate dysregulation of MEKK2 signaling in breast carcinoma (9), colorectal carcinoma (10), and pancreatic ductal adenocarcinoma (8).
- Sun, W. et al. (2003) Mol Cell Biol 23, 2298-308.
- Kesavan, K. et al. (2004) J Cell Physiol 199, 140-8.
- Cheng, J. et al. (2005) Mol Cell Biol 25, 5955-64.
- Cheng, J. et al. (2005) J Biol Chem 280, 13477-82.
- Takeda, A.N. et al. (2014) EMBO J 33, 1784-801.
- Yamashita, M. et al. (2005) Cell 121, 101-13.
- Zhang, D. et al. (2006) EMBO J 25, 97-107.
- Mazur, P.K. et al. (2014) Nature 510, 283-7.
- Mirza, A.A. et al. (2014) Biochim Biophys Acta 1843, 945-54.
- Jiang, L. et al. (2013) Oncol Lett 6, 1333-37.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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