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MDM2 (E3G5I) Rabbit mAb #51541
Filter:
- WB
- IP
Western blot analysis of extracts from SJSA-1, Saos-2 (human), C2C12 (mouse), and MTLn3 (rat) cells, untreated (-) or treated with Nutlin 3a (10 μM, 24 hr; +), using MDM2 (E3G5I) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of MDM2 protein in Saos-2 cells is consistent with the predicted expression pattern.
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 90 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
MDM2 (E3G5I) Rabbit mAb recognizes endogenous levels of total MDM2 protein. Based on the sequence of the epitope, this antibody is expected to detect full-length MDM2, as well as isoforms A, C, alpha, F, G, and 11. This antibody may detect isoform A1. This antibody cross-reacts with a band of unknown origin at approximately 60 kDa.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human MDM2 protein. This antibody recognizes an epitope surrounding Val280 of human MDM2 protein.
Background
MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 (1). Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53 (2-4). Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 (3).
Pathways
Explore pathways related to this product.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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