Render Target: SSR
Render Timestamp: 2024-12-30T23:30:20.787Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-20 06:16:36.938
Product last modified at: 2024-09-13T07:01:28.623Z
Cell Signaling Technology Logo
View Featured Offers >>
1% for the planet logo
PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464
  1. Products /
  2. Primary Antibodies /
  3. MDA-5 (R470) Antibody

MDA-5 (R470) Antibody #4110

We recommend the following alternatives

#

Product Name

5321 MDA-5 (D74E4) Rabbit mAb
Filter:
  • WB
  • IP

Inquiry Info. # 4110

Please see our recommended alternatives.

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 135
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MDA-5 (R470) Antibody detects endogenous levels of total MDA-5 protein.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrouding Arg470 of human MDA-5. Antibody is purified by protein A and peptide affinity chromatography.

    Background

    Antiviral innate immunity depends on the combination of parallel pathways triggered by virus detecting proteins in the Toll-like receptor (TLR) family and RNA helicases, such as Rig-I (retinoic acid-inducible gene I) and MDA-5 (melanoma differentiation-associated antigen 5), which promote the transcription of type I interferons (IFN) and antiviral enzymes (1-3). TLRs and helicase proteins contain sites that recognize the molecular patterns of different virus types, including DNA, single-stranded RNA (ssRNA), double-stranded RNA (dsRNA), and glycoproteins. These antiviral proteins are found in different cell compartments; TLRs (i.e. TLR3, TLR7, TLR8, and TLR9) are expressed on endosomal membranes and helicases are localized to the cytoplasm. Rig-I expression is induced by retinoic acid, LPS, IFN, and viral infection (4,5). Both Rig-I and MDA-5 share a DExD/H-box helicase domain that detects viral dsRNA and two amino-terminal caspase recruitment domains (CARD) that are required for triggering downstream signaling (4-7). Rig-I binds both dsRNA and viral ssRNA that contains a 5'-triphosphate end not seen in host RNA (8,9). Though structurally related, Rig-I and MDA-5 detect a distinct set of viruses (10,11). The CARD domain of the helicases, which is sufficient to generate signaling and IFN production, is recruited to the CARD domain of the MAVS/VISA/Cardif/IPS-1 mitochondrial protein, which triggers activation of NF-κB, TBK1/IKKε, and IRF-3/IRF-7 (12-15).
    MDA-5 (16,17), also named Ifih1 (interferon induced with helicase C domain 1), RH116 (RNA helicase-DEAD box protein 116) (18), and Helicard (19) is found to be induced by interferon. During apoptosis, MDA-5 is cleaved by caspases, separating the helicase and CARD domains (19). MDA-5 is uniquely activated by picornavirus (20) and measles virus (21).
    1. Yoneyama, M. and Fujita, T. (2007) J Biol Chem 282, 15315-8.
    2. Meylan, E. and Tschopp, J. (2006) Mol Cell 22, 561-9.
    3. Thompson, A.J. and Locarnini, S.A. (2007) Immunol Cell Biol 85, 435-45.
    4. Imaizumi, T. et al. (2002) Biochem Biophys Res Commun 292, 274-9.
    5. Zhang, X. et al. (2000) Microb Pathog 28, 267-78.
    6. Yoneyama, M. et al. (2005) J Immunol 175, 2851-8.
    7. Yoneyama, M. et al. (2004) Nat Immunol 5, 730-7.
    8. Hornung, V. et al. (2006) Science 314, 994-7.
    9. Pichlmair, A. et al. (2006) Science 314, 997-1001.
    10. Kato, H. et al. (2006) Nature 441, 101-5.
    11. Childs, K. et al. (2007) Virology 359, 190-200.
    12. Meylan, E. et al. (2005) Nature 437, 1167-72.
    13. Xu, L.G. et al. (2005) Mol Cell 19, 727-40.
    14. Kawai, T. et al. (2005) Nat Immunol 6, 981-8.
    15. Seth, R.B. et al. (2005) Cell 122, 669-82.
    16. Kang, D.C. et al. (2002) Proc Natl Acad Sci U S A 99, 637-42.
    17. Kang, D.C. et al. (2004) Oncogene 23, 1789-800.
    18. Cocude, C. et al. (2003) J Gen Virol 84, 3215-25.
    19. Kovacsovics, M. et al. (2002) Curr Biol 12, 838-43.
    20. Kato, H. et al. (2006) Nature 441, 101-5.
    21. Berghäll, H. et al. (2006) Microbes Infect 8, 2138-44.

    Database Links

    UniProt ID: Q9BYX4


    Entrez-Gene Id: 64135


    限制使用

    除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。

    专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.