R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
MCT4/SLC16A3 (F2K6A) Rabbit mAb #81569
Filter:
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 40 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
MCT4/SLC16A3 (F2K6A) Rabbit mAb recognizes endogenous levels of total MCT4/SLC16A3 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human MCT4/SLC16A3 protein.
Background
MCT4, also known as MOT4 or SLC16A3, is a multi-pass transmembrane monocarboxylate transporter (MCT) protein encoded by the gene SLC16A3 (1,2). MCT4 is reported to be expressed in white (glycolytic) muscle cells (3,4), astrocytes (5), activated macrophages (6), and cancer cells (2). The primary role of MCT4 is lactate exportation, which is adapted to the release of lactate from glycolytic cells (8,9). In the tumor microenvironment, MCT4 mediated lactate secretion promotes M2 macrophage polarization and suppresses T cell function (10). MCT4 inhibition increases the efficacy of immune checkpoint blockade and is a potential target to reverse tumor resistance to immunotherapy (11,12).
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- Liu, T. et al. (2023) Cancer Manag Res 15, 957-975.
- Wilson, M.C. et al. (1998) J Biol Chem 273, 15920-6.
- Pilegaard, H. et al. (1999) Am J Physiol 276, E843-8.
- Pierre, K. and Pellerin, L. (2005) J Neurochem 94, 1-14.
- Tan, Z. et al. (2015) J Biol Chem 290, 46-55.
- Payen, V.L. et al. (2020) Mol Metab 33, 48-66.
- Dimmer, K.S. et al. (2000) Biochem J 350 Pt 1, 219-27.
- Contreras-Baeza, Y. et al. (2019) J Biol Chem 294, 20135-20147.
- Qian, Y. et al. (2023) Cancer Cell 41, 1363-1380.e7.
- Babl, N. et al. (2023) J Immunother Cancer 11, e007349. doi: 10.1136/jitc-2023-007349.
- Yu, T. et al. (2024) Cancer Lett 589, 216824.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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