R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
MCP-1 (F9I9N) Rabbit mAb #23236
Filter:
- WB
- IP
- IF
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 13-15 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:400 - 1:1600 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
MCP-1 (F9I9N) Rabbit mAb recognizes endogenous levels of total MCP-1 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human MCP-1 protein.
Background
Monocyte chemoattractant protein-1 (MCP-1), also known as CCL2, monocyte chemotactic activating factor (MCAF) or glioma-derived chemotactic factor-2 (GDCF-2), is the product of the human JE gene and a member of the family of C-C (or β) chemokines (1-4). The predicted molecular weight of MCP-1 protein is 11-13 kDa, but it may migrate at 20-30 kDa due to glycosylation. MCP-1 is secreted by a variety of cell types in response to pro-inflammatory stimuli and was originally described for its chemotactic activity on monocytes. This activity has led to studies demonstrating its role in diseases characterized by monocyte infiltrates such as psoriasis (5), rheumatoid arthritis (6) and atherosclerosis (7). MCP-1 may also contribute to tumor progression and angiogenesis (8). Signaling by MCP-1 is mediated by the G protein-coupled receptor CCR2 (9).
- Matsushima, K. et al. (1989) J Exp Med 169, 1485-90.
- Furutani, Y. et al. (1989) Biochem Biophys Res Commun 159, 249-55.
- Robinson, E.A. et al. (1989) Proc Natl Acad Sci USA 86, 1850-4.
- Rollins, B.J. et al. (1988) Proc Natl Acad Sci USA 85, 3738-42.
- Gillitzer, R. et al. (1993) J Invest Dermatol 101, 127-31.
- Koch, A.E. et al. (1992) J Clin Invest 90, 772-9.
- Ylä-Herttuala, S. et al. (1991) Proc Natl Acad Sci USA 88, 5252-6.
- Salcedo, R. et al. (2000) Blood 96, 34-40.
- Charo, I.F. et al. (1994) Proc Natl Acad Sci USA 91, 2752-6.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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