R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
MAVS (E8Z7M) Rabbit mAb #83000
Filter:
- WB
- IP
- IHC
- IF
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 75 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
| Immunofluorescence (Frozen) | 1:100 - 1:400 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:400 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #98156.
For a carrier free (BSA and azide free) version of this product see product #98156.
Protocol
Specificity / Sensitivity
MAVS (E8Z7M) Rabbit mAb recognizes endogenous levels of total MAVS protein. Non-specific nuclear staining in mouse brain and apical membrane staining of mouse stomach epithelium were observed by immunohistochemistry.
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala449 of mouse MAVS protein.
Background
The mitochondrial antiviral signaling protein (MAVS, VISA) contributes to innate immunity by triggering IRF-3 and NF-κB activation in response to viral infection, leading to the production of IFN-β (1). The MAVS protein contains an N-terminal CARD domain and a C-terminal mitochondrial transmembrane domain. The MAVS adaptor protein plays a critical and specific role in viral defenses (2). MAVS acts downstream of the RIG-I RNA helicase and viral RNA sensor, leading to the recruitment of IKKε, TRIF, and TRAF6 (3,4). Some viruses have evolved strategies to circumvent these innate defenses by using proteases that cleave MAVS to prevent its mitochondrial localization (5,6).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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