R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Matrin 3 (F4Q5F) Rabbit mAb #45457
Filter:
- WB
- IP
- IF
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 125 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Frozen) | 1:50 - 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:400 - 1:1600 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Matrin 3 (F4Q5F) Rabbit mAb recognizes endogenous levels of total matrin 3 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human matrin 3 protein.
Background
Matrin 3 (MATR3) is a nuclear protein with two zinc finger domains, two RNA recognition motifs, and two intrinsically disordered regions. This protein is able to bind both DNA and RNA sequences and is a critical component of the nuclear matrix (1-4). MATR3 was initially implicated in human disease through its identification in a family with an autosomal dominant form of vocal cord and pharyngeal distal myopathy (5). Since then, exome sequencing has identified over a dozen point mutations in the MATR3 gene that have been linked to familial amyotrophic lateral sclerosis (ALS) and combined ALS/frontotemporal dementia (6-8). Moreover, abnormal MATR3 abundance, localization, and aggregation have been reported in motor neurons of patients with sporadic ALS (9,10).
- Nakayasu, H. and Berezney, R. (1991) Proc Natl Acad Sci USA 88, 10312-6.
- Belgrader, P. et al. (1991) J Biol Chem 266, 9893-9.
- Malik, A.M. et al. (2018) Elife 7, e35977. doi: 10.7554/eLife.35977.
- Gallego-Iradi, M.C. et al. (2019) Lab Invest 99, 1030-1040.
- Feit, H. et al. (1998) Am J Hum Genet 63, 1732-42.
- Senderek, J. et al. (2009) Am J Hum Genet 84, 511-8.
- Johnson, J.O. et al. (2014) Nat Neurosci 17, 664-666.
- Origone, P. et al. (2015) Amyotroph Lateral Scler Frontotemporal Degener 16, 530-1.
- Dreser, A. et al. (2017) Cell Death Differ 24, 1655-1671.
- Tada, M. et al. (2018) Am J Pathol 188, 507-514.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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