R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
MATE1/SLC47A1 (D4C6Z) Rabbit mAb #14550
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 48-52 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
MATE1/SLC47A1 (D4C6Z) Rabbit mAb recognizes endogenous levels of total MATE1 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu505 of human MATE1 protein.
Background
The multidrug and toxin extrusion protein 1 (MATE1, SLC47A1) is a proton-coupled, organic cation antiporter located at the apical membrane of proximal kidney epithelial cells and the canalicular membrane of hepatocytes (1). MATE1 mediates the secretion of organic cations including drugs, toxins, and endogenous metabolites, into bile and urine (2,3). Substrates of MATE1 include multiple therapeutic agents, including metformin, cisplatin, acyclovir, and cephalexin (4,5). Polymorphisms in the corresponding SLC47A1 gene may affect the rate of renal clearance of certain cationic drugs, limiting the therapeutic benefits of these agents (6). Specifically, research studies demonstrate that SLC47A1 allelic variation correlates with differences in renal clearance rates of metformin (7), which may have an effect on the therapeutic impact of this drug in individuals diagnosed with type 2 diabetes (8).
- Otsuka, M. et al. (2005) Proc Natl Acad Sci U S A 102, 17923-8.
- Omote, H. et al. (2006) Trends Pharmacol Sci 27, 587-93.
- Motohashi, H. and Inui, K. (2013) AAPS J 15, 581-8.
- Tanihara, Y. et al. (2007) Biochem Pharmacol 74, 359-71.
- Hume, W.E. et al. (2013) Bioorg Med Chem 21, 7584-90.
- Staud, F. et al. (2013) Int J Biochem Cell Biol 45, 2007-11.
- Christensen, M.M. et al. (2013) Pharmacogenet Genomics 23, 526-34.
- Becker, M.L. et al. (2009) Diabetes 58, 745-9.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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