Render Target: SSR
Render Timestamp:
3/26/2025, 8:45:49 AM EDT
3/26/2025, 12:45:49 PM UTC
Commit: 8d93f7ebe45006d66c127727d817fc3f57c4fe9a
XML generation date: 2025-03-07 13:12:30.891
Product last modified at: 2024-05-30T07:01:29.454Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

MAP4K3 Antibody #9613

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Inquiry Info. # 9613

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    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 101
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MAP4K3 Antibody recognizes endogenous levels of total MAP4K3 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly407 of human MAP4K3 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    The mTORC1 kinase complex is a critical regulator of cell growth (1,2). Its activity is modulated by growth factors and nutrients including amino acids (1,2). MAP4K3 is a mediator between nutrient signal and mTORC1 (1). Research studies suggest that amino acid sufficiency leads to the phosphorylation of Ser170 on MAP4K3, which activates mTORC1 (3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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