Lyn Antibody #2732
Filter:
- WB
- IP
Western blot analysis of extracts from Ramos cells treated with anti-IgM antibody (12 µg/ml for 2 minutes) or untreated, using Lyn Antibody.
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 56 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Lyn Antibody detects endogenous levels of the 56 kDa isoform of Lyn.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human Lyn. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Lyn, one of the Src family members, is predominantly expressed in hematopoietic cells (1). Two tyrosine residues have been reported to play a crucial role in the regulation of protein tyrosine kinases of the Src family. Autophosphorylation of Tyr396 (equivalent to Tyr416 of Src), located in the catalytic domain, correlates with enzyme activation. Csk-mediated phosphorylation of the carboxy-terminal Tyr507 (equivalent to Tyr527 of Src) inactivates the kinase. Tyrosine phosphorylation and activation of Lyn occurs upon association with cell surface receptors such as the B cell Ag receptor (BCR) and CD40 (2-4). Studies using knockout mice have shown that the net effect of Lyn deficiency is to render B cells hypersensitive to BCR stimulation (5-7), suggesting that the most critical role for Lyn in vivo is in the down-regulation of B cell responses. Lyn is also involved in controlling the migration and development of specific B cell populations (8).
- Yamanashi, Y. et al. (1989) Proc. Natl. Acad. Sci. USA 86, 6538-6542.
- Yamanashi, Y. et al. (1991) Science 251, 192-194.
- Burkhardt, A.L. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 7410-7414.
- Ren, C.L. et al. (1994) J. Exp. Med. 179, 673-680.
- Wang, J. et al. (1996) J Exp Med 184, 831-8.
- Chan, V.W. et al. (1997) Immunity 7, 69-81.
- Hibbs, M.L. et al. (1995) Cell 83, 301-11.
- Seo, S. et al. (2001) J Immunol 166, 3710-6.
Pathways
Explore pathways related to this product.
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