R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
LXR-α (E3D9B) Rabbit mAb #79762
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Transfected Only |
| MW (kDa) | 48,52 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
LXR-α (E3D9B) Rabbit mAb recognizes overexpressed levels of total LXR-α protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human LXR-α protein.
Background
Liver X receptors LXR-α and LXR-β are nuclear hormone receptor superfamily members responsible for regulating expression of target genes that control cholesterol transport and metabolism (1). When bound by the oxidized derivatives of cholesterol (oxysterols), activated LXR receptors function as sterol sensors to regulate transcription of the genes involved in the cholesterol homeostasis (1,2). The LXR-α protein is expressed at high levels in rat liver, kidney, intestine, adipose, and spleen; LXR-β is more ubiquitously expressed within rat tissues (1,3). Research studies indicate that glucose binds and upregulates the transcriptional activity of LXR-α and LXR-β (4). LXR-α and LXR-β are putative glucose sensors that integrate glucose metabolism and fatty acid biosynthesis in the liver (4). Additional studies show that female mice deficient in LXR-β develop gallbladder cancer (5). In addition, LXR-β plays a role in protecting dopaminergic neurons in a Parkinson's disease model (6).
- Repa, J.J. et al. (2000) Genes Dev 14, 2819-30.
- Willy, P.J. et al. (1995) Genes Dev 9, 1033-45.
- Teboul, M. et al. (1995) Proc Natl Acad Sci USA 92, 2096-100.
- Mitro, N. et al. (2007) Nature 445, 219-23.
- Gabbi, C. et al. (2010) Proc Natl Acad Sci USA 107, 14763-8.
- Dai, Y.B. et al. (2012) Proc Natl Acad Sci USA 109, 13112-7.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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