R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Lunatic Fringe (D6V2V) Rabbit mAb #66472
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 42 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Lunatic Fringe (D6V2V) Rabbit mAb recognizes endogenous levels of total Lunatic Fringe protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu74 of human Lunatic Fringe protein.
Background
Lunatic Fringe (Beta-1,3-N-acetylglucosaminyltransferase, LFNG) is a single-pass type II Golgi membrane glycosyltransferase that catalyzes the elongation of O-linked fucose residues on EGF-like repeats of Notch signaling molecules. Fucosylation of EGF-like repeats serves to fine-tune Notch ligand-receptor interactions, thereby modulating downstream Notch pathway activity (1). Studies in genetic mouse models have shown that Lunatic Fringe-mediated Notch regulation is critical for somite patterning during vertebrate embryogenesis (2-4). Consistent with this, loss-of-function mutations in human LFNG are associated with spondylocostal dysostoses, a heritable skeletal growth disorder characterized by malformations of the spinal column and thoracic structures (5). Lunatic Fringe continues to modulate Notch signaling postnatally (6), and is implicated as a putative tumor suppressor in multiple Notch-related cancers (7, 8).
- Shimizu, K. et al. (2001) J Biol Chem 276, 25753-8.
- Evrard, Y.A. et al. (1998) Nature 394, 377-81.
- Zhang, N. and Gridley, T. (1998) Nature 394, 374-7.
- Serth, K. et al. (2015) PLoS One 10, e0123776.
- Sparrow, D.B. et al. (2006) Am J Hum Genet 78, 28-37.
- Mukherjee, S. et al. (2014) J Immunol 192, 996-1003.
- Zhang, S. et al. (2014) Neoplasia 16, 158-67.
- Zhang, S. et al. (2015) Oncogene , .
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For Research Use Only. Not For Use In Diagnostic Procedures.
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