R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
LTβR (E5I6X) Rabbit mAb #57560
Filter:
- WB
- IP
- IF
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 45-70 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:200 - 1:800 |
| Flow Cytometry (Fixed/Permeabilized) | 1:400 - 1:600 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
LTβR (E5I6X) Rabbit mAb recognizes endogenous levels of total LTβR protein. This antibody is expected to recognize multiple isoforms of LTβR protein. This antibody cross-reacts with an unidentified protein of 20 kDa and 30 kDa in some cell extracts.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg428 of human LTβR protein.
Background
Lymphotoxin β receptor (LTβR), also known as tumor necrosis factor receptor superfamily member 3 (TNFRSF3) and tumor necrosis factor receptor type III (TNF-RIII), is a type I membrane receptor expressed by stromal cells, including endothelial, mesenchymal, and epithelial cells, and by myeloid cells, such as dendritic cells and macrophages (1-4). When lymphotoxin α (LTα) is co-expressed with lymphotoxin β (LTβ) that possesses a transmembrane domain, it forms a cell surface-bound heterotrimer (LTα1β2) that exclusively binds to LTβR (5,6). The LTα1β2/LTβR pathway is required for the normal development of lymph nodes and Peyer’s patches (7,8). Disruption of the LTα1β2/LTβR pathway results in impaired protection from viral and bacterial infections (9,10). LTβR is also a receptor for LIGHT/TNFSF14 (11,12). LTβR signaling through LIGHT/TNFSF14 regulates self-renewal and differentiation of hematopoietic and leukemic stem cells (13). In cancer cells, LTβR signaling via TRAF molecules promotes apoptosis (14-16). Agonistic antibodies to LTβR trigger cancer cell death and suppress tumor growth in vivo (17).
- Murphy, M. et al. (1998) Cell Death Differ 5, 497-505.
- Browning, J.L. and French, L.E. (2002) J Immunol 168, 5079-87.
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- Ehlers, S. et al. (2003) J Immunol 170, 5210-8.
- Wege, A.K. et al. (2014) Innate Immun 20, 461-70.
- Maeda, T. et al. (2018) J Immunol 201, 202-214.
- Schneider, K. et al. (2004) Immunol Rev 202, 49-66.
- Höpner, S.S. et al. (2021) Nat Commun 12, 1065.
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- Force, W.R. et al. (1997) J Biol Chem 272, 30835-40.
- Rooney, I.A. et al. (2000) J Biol Chem 275, 14307-15.
- Lukashev, M. et al. (2006) Cancer Res 66, 9617-24.
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