LshCas13a/C2c2 (Leptotrichia shahii) (7D1-H7) Mouse mAb #43731
Filter:
- WB
- IF
Supporting Data
REACTIVITY | All |
SENSITIVITY | Transfected Only |
MW (kDa) | 166 |
Source/Isotype | Mouse IgG1 |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunofluorescence (Immunocytochemistry) | 1:50 - 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
LshCas13a/C2c2 (Leptotrichia shahii) (7D1-H7) Mouse mAb recognizes transfected levels of total LshCas13a/C2c2 (Leptotrichia shahii) protein. This antibody may detect several non-specific bands of unknown origin in rat cell lines.
Species Reactivity:
All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of LshCas13a/C2c2 (Leptotrichia shahii) protein.
Background
CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins) are RNA-guided nuclease effectors that are utilized for precise genome editing in mammalian systems (1). Cas13 proteins are members of the Class 2 CRISPR-Cas system. Class 2 CRISPR systems, such as the well characterized Cas9, rely on single-component effector proteins to mediate DNA/RNA interference rather than the multi-Cas protein complexes that belong to the Class 1 systems (2). Evolutionarily, CRISPR-Cas proteins act as RNA-guided antiviral and anti-plasmid that protect prokaryotes from phage-mediated infections. RNA-guided CRISPR-Cas proteins can act on both DNA and RNA. Cas13 proteins act as RNA-guided RNA nucleases, disrupting phage infection by RNA cleavage. The CRISPR-Cas system is being used for nuclease-based gene-editing. Since the Cas13 proteins act on RNA rather than DNA, Cas13 proteins, coupled with the appropriate guide RNA, can be used to target specific RNA substrates, potentially replacing or complementing RNA interference approaches that interfere with transcript levels (3). Several Cas13 bacterial orthologs have been characterized for CRISPR-mediated mammalian RNA editing, including LshCas13a/C2c2 and PspCas13b derived from Leptotrichia shahii and Prevotella sp. P5–125, respectively (4-6).
- Cong, L. et al. (2013) Science 339, 819-23.
- Horvath, P. and Barrangou, R. (2010) Science 327, 167-70.
- Wang, Q. et al. (2019) Adv Sci (Weinh) 6, 1901299.
- East-Seletsky, A. et al. (2016) Nature 538, 270-273.
- Smargon, A.A. et al. (2017) Mol Cell 65, 618-630.e7.
- Cox, D.B.T. et al. (2017) Science 358, 1019-1027.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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