LRBA Antibody #86622
Filter:
- WB
- IP
Supporting Data
REACTIVITY | H |
SENSITIVITY | Endogenous |
MW (kDa) | 320 |
SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
LRBA Antibody recognizes endogenous levels of total LRBA protein. This antibody detects a 130 kDa band of unknown origin in some cell lines.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human LRBA protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
LRBA (lipopolysaccharide responsive beige-like anchor protein) is a 300 kDa intracellular protein that is a member of the PH-BEACH-WD40 protein family (1-3). Its BEACH domain contains an A-kinase anchoring protein (AKAP) motif, known to have an affinity for signaling enzymes (1,3). LRBA has been found to be overexpressed in several different cancers, such as renal, pancreas, colorectal, and lung (2). LRBA deficiency, caused by various mutations, can cause common variable immune deficiency (CVID) as well as other immune dysregulations (3,5). LRBA plays an immunoregulatory role to CTLA-4, protecting it from being degraded within lysosomes (4,6). In LRBA deficiency, CTLA-4 is synthesized normally, but is not delivered to Rab GTPase recycling compartments, instead resulting in enhanced lysosome degradation (4,6,7).
- Wang, J.W. et al. (2001) J Immunol 166, 4586-95.
- Wang, J.W. et al. (2004) Oncogene 23, 4089-97.
- Lopez-Herrera, G. et al. (2012) Am J Hum Genet 90, 986-1001.
- Lo, B. et al. (2015) Science 349, 436-40.
- Gámez-Díaz, L. et al. (2016) J Allergy Clin Immunol 137, 223-230.
- Hou, T.Z. et al. (2017) Blood 129, 1458-1468.
- Janman, D. et al. (2021) Immunology 164, 106-119.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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