R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
LOXL2 (E3P7Y) Rabbit mAb #99680
Filter:
- WB
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 100 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| IHC Leica Bond | 1:400 - 1:1600 |
| Immunohistochemistry (Paraffin) | 1:800 - 1:3200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #72903.
For a carrier free (BSA and azide free) version of this product see product #72903.
Protocol
Specificity / Sensitivity
LOXL2 (E3P7Y) Rabbit mAb recognizes endogenous levels of total LOXL2 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human LOXL2 protein.
Background
Lysyl oxidase-like 2 (LOXL2) is a secreted copper-dependent amine oxidase and a member of the lysyl oxidase family (1). It primarily catalyzes the oxidation of lysine (or hydroxylysine) residues in collagen and elastin to form peptidyl aldehyde derivatives (1). These modifications are required for further cross-linking of target proteins to enhance extracellular matrix (ECM) stiffness. LOXL2 has been associated with progression and metastases of many cancer types, including breast cancer and fibrotic disease, all through active remodeling of the ECM (2-5). Additionally, within the cell, LOXL2 has been shown to play a role in EMT, cell polarity, and transcriptional repression (6-8). LOXL2 has become an attractive target for therapeutic intervention in cancer treating, blocking metastasis, and halting fibrosis (9-10).
- Xiao, Q. and Ge, G. (2012) Cancer Microenviron 5, 261-73.
- Wang, C. et al. (2019) Neoplasia 21, 413-27.
- Lewis, M.A. et al. (1985) Med Pediatr Oncol 13, 97-100.
- Du, X.G. and Zhu, M.J. (2018) Onco Targets Ther 11, 2699-2708.
- Tian, J. et al. (2019) Onco Targets Ther 12, 8947-54.
- Cebrià-Costa, J.P. et al. (2020) Oncogene 39, 79-121.
- Wen, X. et al. (2018) Int J Mol Med 42, 3530-41.
- Moreno-Bueno, G. et al. (2011) EMBO Mol Med 3, 528-44.
- Puente, A. et al. (2019) Int J Mol Sci 20, pii: E1634. doi: 10.3390/ijms20071634.
- Trackman, P.C. (2016) Expert Opin Ther Targets 20, 935-45.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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