R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
LOXL2 (E3P7Y) Rabbit mAb (BSA and Azide Free) #72903
Filter:
- WB
- IHC
Western blot analysis of extracts from BT-549 and HCT-15 cells using LOXL2 (E3P7Y) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). The differential in LOXL2 expression between BT-549 and HCT-15 cells is consistent with their reported molecular expression profiles (www.depmap.org), confirming specificity of the antibody for LOXL2 (E3P7Y) Rabbit mAb. Data were generated using the standard formulation of this product.
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 100 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- Related Products
Product Information
Product Usage Information
This product is the carrier free version of product #99680. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.
For standard formulation of this product see product #99680
For standard formulation of this product see product #99680
Storage
Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Specificity / Sensitivity
LOXL2 (E3P7Y) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total LOXL2 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human LOXL2 protein.
Background
Lysyl oxidase-like 2 (LOXL2) is a secreted copper-dependent amine oxidase and a member of the lysyl oxidase family (1). It primarily catalyzes the oxidation of lysine (or hydroxylysine) residues in collagen and elastin to form peptidyl aldehyde derivatives (1). These modifications are required for further cross-linking of target proteins to enhance extracellular matrix (ECM) stiffness. LOXL2 has been associated with progression and metastases of many cancer types, including breast cancer and fibrotic disease, all through active remodeling of the ECM (2-5). Additionally, within the cell, LOXL2 has been shown to play a role in EMT, cell polarity, and transcriptional repression (6-8). LOXL2 has become an attractive target for therapeutic intervention in cancer treating, blocking metastasis, and halting fibrosis (9-10).
- Xiao, Q. and Ge, G. (2012) Cancer Microenviron 5, 261-73.
- Wang, C. et al. (2019) Neoplasia 21, 413-27.
- Lewis, M.A. et al. (1985) Med Pediatr Oncol 13, 97-100.
- Du, X.G. and Zhu, M.J. (2018) Onco Targets Ther 11, 2699-2708.
- Tian, J. et al. (2019) Onco Targets Ther 12, 8947-54.
- Cebrià-Costa, J.P. et al. (2020) Oncogene 39, 79-121.
- Wen, X. et al. (2018) Int J Mol Med 42, 3530-41.
- Moreno-Bueno, G. et al. (2011) EMBO Mol Med 3, 528-44.
- Puente, A. et al. (2019) Int J Mol Sci 20, pii: E1634. doi: 10.3390/ijms20071634.
- Trackman, P.C. (2016) Expert Opin Ther Targets 20, 935-45.
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