Render Target: SSR
Render Timestamp: 2024-10-24T19:41:25.456Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-09-30 01:53:58.697
Product last modified at: 2024-09-30T08:02:06.060Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

LMAN1 (E2B6H) Rabbit mAb #13974

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 53
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    LMAN1 (E2B6H) Rabbit mAb recognizes endogenous levels of total LMAN1 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu366 of human LMAN1 protein.

    Background

    Mannose-binding lectin-1 (LMAN1, ERGIC-53) is a type I transmembrane lectin protein localized to the intermediate compartment between the endoplasmic reticulum and the Golgi body (ERGIC) (1). Interaction between the LMAN1 protein and MCFD2 forms an ERGIC cargo receptor that delivers proteins from the ER to the Golgi body (2,3). The LMAN1 protein contains an amino-terminal carbohydrate recognition domain (CRD) that binds target glycoproteins, a membrane proximal oligomerization domain required for cargo transport, a single transmembrane segment, and short cytoplasmic tail (3-5). LMAN1 functions as a cargo receptor responsible for transport of glycoproteins from the ER to ERGIC and Golgi body. Target proteins include coagulation factors V and VIII, cathepsin C, cathepsin Z, and α1-antitrypsin (6-8). Mutations in the corresponding LMAN1 gene can result in combined factors FV and FVIII deficiency, an autosomal recessive disorder characterized by spontaneous bleeding (9). Inactivating frameshift mutations in LMAN1 are found at high frequency in colorectal tumors with microsatellite instability and may contribute to tumorigenesis (10).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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