LIMP-2/SCARB2 (E2Z5F) Rabbit mAb #27960
- WB
- IP
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 54, 80 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Frozen) | 1:200 - 1:800 |
| Immunofluorescence (Immunocytochemistry) | 1:50 - 1:100 |
Storage
For a carrier free (BSA and azide free) version of this product see product #69463.
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly467 of mouse LIMP-2/SCARB2 protein.
Background
Lysosomal integral membrane protein type 2 (LIMP-2, also known as SCARB2) is a lysosomal membrane glucoprotein encoded by the SCARB2 gene. As a lysosome-enriched protein, LIMP-2/SCARB2 contributes to endosomal and lysosomal transport and function in the cell. β-glucocerebrosidase (GBA), a lysosomal enzyme defective in Gaucher disease and genetically linked to Parkinson’s disease, is targeted to lysosomes by binding directly to LIMP-2/SCARB2 via a mannose 6-phosphate-independent pathway (1-3). LIMP-2/SCARB2 may also play a role in the transport of cholesterol from the lysosome to the membrane, an important function as cholesterol contributes to various biophysical properties of cell membranes and may contribute to various diseases (4,5). Interestingly, SCARB2 is upregulated in neurodegenerative diseases, including Lewy body disease (6), and genetic variants in the gene encoding SCARB2 are linked to Parkinson’s disease (7), suggesting that altered lysosomal function may contribute to neuropathology in these diseases. LIMP-2/SCARB2 also serves as a receptor for enterovirus 71 and coxsackieviruses (8).
- Reczek, D. et al. (2007) Cell 131, 770-83.
- Blanz, J. et al. (2015) Traffic 16, 1127-36.
- Riboldi, G.M. and Di Fonzo, A.B. (2019) Cells 8, 364.
- Brown, M.S. et al. (2018) Annu Rev Biochem 87, 783-807.
- Heybrock, S. et al. (2019) Nat Commun 10, 3521.
- Pérez-Roca, L. et al. (2019) Neurosci Lett 706, 164-168.
- Hopfner, F. et al. (2013) Mov Disord 28, 538-40.
- Yamayoshi, S. et al. (2009) Nat Med 15, 798-801.
限制使用
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