R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
LIME1 (E3M3B) Rabbit mAb #72099
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 30 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
LIME1 (E3M3B) Rabbit mAb recognizes endogenous levels of total LIME1 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro211 of human LIME1 protein.
Background
Lck-interacting transmembrane adaptor 1 (LIME1) is a type III transmembrane adaptor protein that participates in lymphocyte signaling and is predominantly expressed in T lineage cells and plasma cells (1,2). LIME1 also harbors a palmitoylation motif for targeting to lipid rafts and potential tyrosine phosphorylation sites that are engaged upon CD4 and CD8 cross-linking (1). Research studies have demonstrated that in response to T cell receptor (TCR) engagement, LIME1 expression is upregulated, undergoes tyrosine phosphorylation, and associates with numerous signaling proteins such as Lck, GADS, and Vav. Collectively, these observations point toward a role for LIME1 in the regulation of T cell activation and adhesion (3,4).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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