R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
LDHB (E8J8T) Rabbit mAb #56298
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 37 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
LDHB (E8J8T) Rabbit mAb recognizes endogenous levels of total LDHB protein. This antibody does not cross-react with LDHA or LDHC protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala13 of human LDHB protein.
Background
Lactate dehydrogenase (LDH) catalyzes the reversible conversion between pyruvate and lactate. LDH is a tetramer composed of various combinations of LDHA subunit and LDHB subunit to form five different isozymes. LDHA has a higher affinity for pyruvate and preferentially catalyzes the conversion of pyruvate to lactate. LDHA levels are upregulated in many cancers. On the other hand, LDHB has a higher affinity for lactate and preferentially catalyzes the conversion of lactate to pyruvate, enabling cells to use lactate as a nutrient (1-3). Studies show that LDHA/LDHB deficiency suppresses glycolysis and ATP production, inhibiting STING signaling and antitumor immune responses mediated by dendritic cells (4). In addition, acetylation of LDHB inhibits its activity, reduces hepatic lactate clearance, and promotes the progression of non-alcoholic fatty liver disease (NAFLD) (5).
- Doherty, J.R. and Cleveland, J.L. (2013) J Clin Invest 123, 3685-92.
- Hong, S.M. et al. (2019) J Biol Chem 294, 7810-7820.
- Urbańska, K. and Orzechowski, A. (2019) Int J Mol Sci 20, 2085. doi: 10.3390/ijms20092085.
- Hu, Z. et al. (2023) J Clin Invest 133, e166031. doi: 10.1172/JCI166031.
- Wang, T. et al. (2021) J Hepatol 74, 1038-1052.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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