R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
LCN2 (D4M8L) Rabbit mAb #44058
Filter:
- WB
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 22 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:3000 - 1:12000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
LCN2 (D4M8L) Rabbit mAb recognizes endogenous levels of total LCN2 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala73 of human LCN2 protein.
Background
Lipocalin 2 (LCN2), also known as neutrophil gelatinase-associated lipocalin (NGAL), belongs to the lipocalin family. Proteins in this family exhibit considerable sequence diversity, but share a highly conserved structure composed of an eight-stranded antiparallel beta-barrel that encloses an internal ligand-binding site (1,2). LCN2 is considered as an iron transporter. LCN2 can bind bacterial siderophores and inhibits bacterial growth by iron depletion (3). It can also bind endogenous compounds like catechol in complex with iron (4). LCN2 was originally identified in neutrophils, and its expression was induced in many other tissues like kidney and liver in response to various pathologic states, such as infection, ischemia, and acute kidney injury. LCN2 is considered a biomarker for conditions like ischemic stroke, acute kidney injury, inflammatory and metabolic diseases (5-7).
- Flower, D.R. (1996) Biochem J 318 ( Pt 1), 1-14.
- Grzyb, J. et al. (2006) J Plant Physiol 163, 895-915.
- Goetz, D.H. et al. (2002) Mol Cell 10, 1033-43.
- Bao, G. et al. (2010) Nat Chem Biol 6, 602-9.
- Haase-Fielitz, A. et al. (2014) Ann Clin Biochem 51, 335-51.
- Abella, V. et al. (2015) Biomarkers 20, 565-71.
- Hochmeister, S. et al. (2016) PLoS One 11, e0154797.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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