R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
LASS1 (F1D4P) Rabbit mAb #37923
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 29 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
LASS1 (F1D4P) Rabbit mAb recognizes endogenous levels of total LASS1 protein. This antibody does not cross-react with LASS2 or LASS6 and, based on homology, is not predicted to cross-react with other LASS family members.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp324 of human LASS1 protein.
Background
The mammalian ceramide synthases (CerS), also known as longevity assurance homologues (LASS), are a family of proteins that catalyze the formation of ceramide from acyl-CoA and sphingosine as part of the ceramide salvage pathway (1). Sphingosine derived from the lysosomal breakdown of complex glycosphingolipids can be delivered to the endoplasmic reticulum for LASS-dependent ceramide production (1). LASS family members exhibit preferences for different acyl-CoA fatty acid chain lengths (2-6) and have distinct tissue expression patterns, with notable enrichment of LASS1 in brain, LASS2 in liver and kidney, LASS3 in testis and skin, and LASS6 in intestine (6,7). Similarly, LASS proteins may function as tumor suppressors or promoters depending on tumor origin and metabolic context (8-11).
- Kitatani, K. et al. (2008) Cell Signal 20, 1010-8.
- Venkataraman, K. et al. (2002) J Biol Chem 277, 35642-9.
- Riebeling, C. et al. (2003) J Biol Chem 278, 43452-9.
- Mizutani, Y. et al. (2005) Biochem J 390, 263-71.
- Mizutani, Y. et al. (2006) Biochem J 398, 531-8.
- Laviad, E.L. et al. (2008) J Biol Chem 283, 5677-84.
- Mizutani, Y. et al. (2008) J Lipid Res 49, 2356-64.
- Schiffmann, S. et al. (2009) Carcinogenesis 30, 745-52.
- Xing, J. and Yi, J. (2021) J Ovarian Res 14, 117.
- Garcia-Vallicrosa, C. et al. (2023) Int J Mol Sci 24, 15668. doi: 10.3390/ijms242115668.
- Huang, Y. et al. (2024) Cancer Cell Int 24, 87.
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