Lamin B1 (119D5-F1) Mouse mAb #68591
Filter:
- IF
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Mouse IgG1 |
Application Key:
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Immunocytochemistry) | 1:400 - 1:1600 |
Storage
Supplied at 1 mg/mL in PBS containing 0.09% sodium azide. Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present, but will not interfere with antibody performance. This product is stable for 36 months when stored at -20C.
Protocol
Specificity / Sensitivity
Lamin B1 (119D5-F1) Mouse mAb recognizes endogenous levels of total Lamin B1 by immunofluroescence. Testing by immunofluorescence was validated for human cells. Reactivity is expected in other species, including mouse and rat.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with purified rat liver lamins.
Background
Lamins are nuclear membrane structural components that are important in maintaining normal cell functions, such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamins have been subdivided into types A and B. Type-A lamins consist of lamin A and C, which arise from alternative splicing of the lamin A gene LMNA. Lamin A and C are cleaved by caspases into large (41-50 kDa) and small (28 kDa) fragments, which can be used as markers for apoptosis (4,5). Type-B lamins consist of lamin B1 and B2, encoded by separate genes (6-8). Lamin B1 is also cleaved by caspases during apoptosis (9). Research studies have shown that duplication of the lamin B1 gene LMNB1 is correlated with pathogenesis of the neurological disorder adult-onset leukodystrophy (10).
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- Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
- Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
- Rao, L. et al. (1996) J Cell Biol 135, 1441-55.
- Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
- Biamonti, G. et al. (1992) Mol Cell Biol 12, 3499-506.
- Lin, F. and Worman, H.J. (1995) Genomics 27, 230-6.
- Pollard, K.M. et al. (1990) Mol Cell Biol 10, 2164-75.
- Chandler, J.M. et al. (1997) Biochem J 322 ( Pt 1), 19-23.
- Padiath, Q.S. et al. (2006) Nat Genet 38, 1114-23.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer (whether the buyer is an academic or for-profit entity), for Immunocytochemistry, high content screening (HCS) analysis, or flow cytometry applications. The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) resale, whether or not such product or its components are resold for use in research; or for any other commercial purpose. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
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