R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
LAMC1 (E9J7Y) Rabbit mAb #57886
Filter:
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 220-250 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
LAMC1 (E9J7Y) Rabbit mAb recognizes endogenous levels of total LAMC1 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly44 of human LAMC1 protein.
Background
Laminins are a family of proteins important for maintaining cellular basement membrane (BM) structure and function (1). Laminins exist as heterotrimers of alpha (LAMA), beta (LAMB), and gamma (LAMC) chains. LAMC1 (laminin gamma-1) is a ubiquitously expressed gamma chain, and has three distinct functional domain structures. The N-terminal LN domain binds to specific alpha and beta chains to form a unique laminin heterotrimer. The LE domain mediates interactions with nidogens, while the C-terminal coil-coil fragment is necessary for agrin association. Collectively, these interactions are critical for stabilization and function of the BM (1). Genetic ablation of LAMC1 has demonstrated a critical role for this protein in embryo implantation, lung and kidney development, and neuronal Schwann cell myelination and regeneration, in addition to Trypanosome infection (2-5). Upregulation of LAMC1 has also been associated with tumor progression in multiple tumor types (6-9), possibly by creating a BM environment that is favorable for cancer cell metastasis and invasion.
- Yurchenco, P.D. (2011) Cold Spring Harb Perspect Biol 3, a004911.
- Ramathal, C. et al. (2011) J Biol Chem 286, 19860-71.
- Willem, M. et al. (2002) Development 129, 2711-22.
- Chen, Z.L. and Strickland, S. (2003) J Cell Biol 163, 889-99.
- Nde, P.N. et al. (2012) Front Immunol 3, 337.
- Liétard, J. et al. (1997) Am J Pathol 151, 1663-72.
- Nishikawa, R. et al. (2014) Int J Oncol 45, 401-10.
- Huang, S.X. et al. (2016) Int J Oncol 49, 217-24.
- Kashima, H. et al. (2015) Gynecol Oncol 139, 338-44.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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