R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Kv4.2 (D3E7G) Rabbit mAb #74748
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 70 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Kv4.2 (D3E7G) Rabbit mAb recognizes endogenous levels of total Kv4.2 protein. This antibody does not cross-react with Kv4.1 and Kv4.3 proteins.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Kv4.2 protein.
Background
Kv4.2 is a voltage-gated potassium channel that belongs to the Shal-related subfamily. Kv4.2 mediates K+ transport in excitable membranes primarily in the brain, where it regulates neuronal excitability, synaptic plasticity, and the circadian rhythm of locomotor activity (1-6). In rodent heart, Kv4.2 mediates the transient outward current (Ito), which contributes to early repolarization and the cardiac action potential (7). Kv4.2 can form homotetramers or heterotetramers with other members of the Shal-related subfamily. Interaction with modulating β subunits such as KChIP family proteins modulates Kv4.2 expression at cell surface and its channel activity (8-10).
- Serôdio, P. and Rudy, B. (1998) J Neurophysiol 79, 1081-91.
- Shibata, R. et al. (2000) J Neurosci 20, 4145-55.
- Zhao, C. et al. (2011) Hippocampus 21, 288-97.
- Granados-Fuentes, D. et al. (2012) J Neurosci 32, 10045-52.
- Labno, A. et al. (2014) PLoS One 9, e84086.
- Yao, J.J. et al. (2016) J Biol Chem 291, 17369-81.
- Yeola, S.W. and Snyders, D.J. (1997) Cardiovasc Res 33, 540-7.
- Kim, L.A. et al. (2004) J Biol Chem 279, 5549-54.
- Jerng, H.H. et al. (2004) Biophys J 87, 2380-96.
- Kitazawa, M. et al. (2014) J Biol Chem 289, 17597-609.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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