R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
KLF2 (E7K8Y) Rabbit mAb #51221
Filter:
- WB
- IP
- IHC
- IF
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 42 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
| Immunofluorescence (Immunocytochemistry) | 1:800 - 1:1600 |
| Flow Cytometry (Fixed/Permeabilized) | 1:100 - 1:400 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #96420.
For a carrier free (BSA and azide free) version of this product see product #96420.
Protocol
Specificity / Sensitivity
KLF2 (E7K8Y) Rabbit mAb recognizes endogenous levels of total KLF2 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to full-length human KLF2 protein.
Background
Krüppel-like factors (KLFs) are a family of zinc finger-containing DNA-binding transcriptional regulators that share homology with the Drosophila melanogaster Krüppel protein that regulates body segmentation. Seventeen mammalian KLFs have been identified so far and they regulate diverse biological processes, including development, differentiation, proliferation, growth, survival, and external stress responses. Alterations in their functions have been linked to many human diseases, including metabolic diseases and cancer (1-3). KLF2 is a key regulator in the differentiation and function of immune cells and vascular endothelial cells. KLF2 suppresses NF-κB-mediated gene expression and plays important roles in inflammatory response (4-7).
- McConnell, B.B. and Yang, V.W. (2010) Physiol Rev 90, 1337-81.
- Tetreault, M.P. et al. (2013) Nat Rev Cancer 13, 701-13.
- Bialkowska, A.B. et al. (2017) Development 144, 737-754.
- Jha, P. and Das, H. (2017) Int J Mol Sci 18, 2383. doi: 10.3390/ijms18112383.
- Turpaev, K.T. (2020) Biochemistry (Mosc) 85, 54-67.
- Wittner, J. and Schuh, W. (2021) Vaccines (Basel) 9, 1171. doi: 10.3390/vaccines9101171.
- Dabravolski, S.A. et al. (2022) Biomedicines 10, 254. doi: 10.3390/biomedicines10020254.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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