Render Target: SSR
Render Timestamp: 2025-01-09T19:39:24.247Z
Commit: 199712eb9daea12d88cc0e67894a8a09f475f8cb
XML generation date: 2024-09-30 01:56:06.585
Product last modified at: 2025-01-01T09:02:56.275Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. KIR2DL3 (D8L3D) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

KIR2DL3 (D8L3D) Rabbit mAb #60040

Filter:
  • F

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 60
    Source/Isotype Rabbit IgG
    Application Key:
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Flow Cytometry (Live) 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    KIR2DL3 (D8L3D) Rabbit mAb recognizes endogenous levels of total KIR2DL3 protein. This antibody weakly cross-reacts with KIR2DL2 proteins in over-expression cell lines.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala173 of human KIR2DL3 protein.

    Background

    Killer cell immunoglobulin-like receptors (KIRs) are type 1 transmembrane glycoproteins expressed by natural killer (NK) cells and subsets of CD4, CD8, and γδ T cells (1-5). Analogous to the diversity of their human leukocyte antigen class I (HLA class I) ligands, the KIR genes are polymorphic and the content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (6,7). The KIR proteins are characterized by the number of extracellular immunoglobulin-superfamily domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain (8-10). KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM) (10), while KIR proteins with the short cytoplasmic domain lack an ITIM and instead transduce activating signals (11,12). KIR proteins play an important role in the regulation of the immune response. Combinations of KIR and HLA class I variants influence susceptibility to autoimmunity and infectious disease, as well as outcomes of haematopoietic stem cell transplantation (12-14).

    KIR2DL3, also referred to as CD158b, interacts with HLA-C alleles (HLA-Cw1, HLA-Cw3, and HLA-Cw7). Upon receptor ligand interaction, KIR2DL3 inhibits the activity of NK cells thus preventing target cell lysis (15-17).
    1. Young, N.T. et al. (2001) J Immunol 166, 3933-41.
    2. Battistini, L. et al. (1997) J Immunol 159, 3723-30.
    3. Björkström, N.K. et al. (2012) Blood 120, 3455-65.
    4. Remtoula, N. et al. (2008) J Immunol 180, 2767-71.
    5. Béziat, V. et al. (2017) Immunology 150, 248-264.
    6. Uhrberg, M. et al. (1997) Immunity 7, 753-63.
    7. Shilling, H.G. et al. (2002) J Immunol 168, 2307-15.
    8. Fan, Q.R. et al. (2001) Nat Immunol 2, 452-60.
    9. Boyington, J.C. et al. (2000) Nature 405, 537-43.
    10. Vivian, J.P. et al. (2011) Nature 479, 401-5.
    11. Stewart, C.A. et al. (2005) Proc Natl Acad Sci USA 102, 13224-9.
    12. Ivarsson, M.A. et al. (2014) Front Immunol 5, 184.
    13. Kulkarni, S. et al. (2008) Semin Immunol 20, 343-52.
    14. Martin, M.P. and Carrington, M. (2013) Immunol Rev 254, 245-64.
    15. Colonna, M. et al. (1993) Proc Natl Acad Sci U S A 90, 12000-4.
    16. Winter, C.C. et al. (1998) J Immunol 161, 571-7.
    17. Moesta, A.K. et al. (2008) J Immunol 180, 3969-79.

    Database Links

    UniProt ID: P43628


    Entrez-Gene Id: 3804


    限制使用

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