R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Kinectin 1 (D5F7J) Rabbit mAb #13243
Filter:
- WB
- IP
- IF
Supporting Data
| REACTIVITY | H Mk Dg |
| SENSITIVITY | Endogenous |
| MW (kDa) | 160 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
- Dg-Dog
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Kinectin 1 (D5F7J) Rabbit mAb recognizes endogenous levels of total kinectin 1 protein.
Species Reactivity:
Human, Monkey, Dog
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val186 of human kinectin 1 protein.
Background
Kinectin 1 (KTN1) is an endoplasmic reticulum (ER)-enriched integral membrane protein that may be involved in the formation of ER sheets (reviewed in 1). Kinectin 1 binds the microtubule motor protein kinesin and acts as a membrane anchor for kinesin-based organelle trafficking (2). The interaction of kinesin with kinectin 1 has been shown to affect ER-supported focal adhesion assembly (3). Kinectin 1 has also been implicated in translation elongation, as an anchor for the elongation factor complex to the ER (4,5). Research investigators have shown that kinectin 1 expression is altered in multiple human pathologies, including breast cancer (6), hepatocellular carcinoma (HCC) (7), Parkinson's disease (8), and the autoimmune syndrome Behçet's disease (BD) (9,10).
- Lin, S. et al. (2012) Int J Biochem Cell Biol 44, 1436-43.
- Kumar, J. et al. (1995) Science 267, 1834-7.
- Zhang, X. et al. (2010) J Cell Sci 123, 3901-12.
- Ong, L.L. et al. (2003) J Biol Chem 278, 32115-23.
- Ong, L.L. et al. (2006) J Biol Chem 281, 33621-34.
- Sutton, C.W. et al. (2010) J Proteome Res 9, 3891-902.
- Wang, H.C. et al. (2004) Biochem Cell Biol 82, 321-7.
- van Dijk, K.D. et al. (2012) Brain Pathol 22, 485-98.
- Lu, Y. et al. (2005) Arthritis Res Ther 7, R1133-9.
- Feng, X.G. et al. (2007) Clin Exp Rheumatol 25, S80-5.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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