Kindlin-2 Antibody #13562
Filter:
- WB
Western blot analysis of extracts from HeLa, Saos-2, and MCF7 cells using Kindlin-2 Antibody.
Supporting Data
| REACTIVITY | H M Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 77 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- Mk-Monkey
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Kindlin-2 Antibody recognizes endogenous levels of total kindlin-2 protein. This antibody does not cross-react with kindlin-1 or kindlin-3 proteins.
Species Reactivity:
Human, Mouse, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn502 of human kindlin-2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The kindlin family of focal adhesion proteins is involved in multiple biological processes, including integrin signaling, adhesion, migration, angiogenesis, differentiation, and mitotic spindle formation (1,2). Kindlin family members 1, 2, and 3 (FERM1, FERM2, and URP2) are differentially expressed in tissues. Kindlin-1 is primarily expressed in epithelial cells, kindlin-2 is ubiquitously expressed, and kindlin-3 expression is restricted to the hematopoietic system (3).
Kindlin-2 regulates integrin β3 mediated adhesion and migration (4,5) and contributes to Wnt signaling through interaction with β-catenin and activation of Wnt target genes (6).
Research studies indicate that kindlin-2 expression in pancreatic and gastric cancers may be indicative of poor prognosis (7,8) and that kindlin-2 may support invasiveness in breast cancer (9). Additional studies show that expression of kindlin-2 in prostate cancer cells can protect cells from cisplatin-induced cell death (10).
Kindlin-2 regulates integrin β3 mediated adhesion and migration (4,5) and contributes to Wnt signaling through interaction with β-catenin and activation of Wnt target genes (6).
Research studies indicate that kindlin-2 expression in pancreatic and gastric cancers may be indicative of poor prognosis (7,8) and that kindlin-2 may support invasiveness in breast cancer (9). Additional studies show that expression of kindlin-2 in prostate cancer cells can protect cells from cisplatin-induced cell death (10).
- Ye, F. and Petrich, B.G. (2011) Curr Opin Hematol 18, 356-60.
- Meves, A. et al. (2009) Trends Cell Biol 19, 504-13.
- Karaköse, E. et al. (2010) J Cell Sci 123, 2353-6.
- Ma, Y.Q. et al. (2008) J Cell Biol 181, 439-46.
- Bledzka, K. et al. (2010) J Biol Chem 285, 30370-4.
- Yu, Y. et al. (2012) EMBO Rep 13, 750-8.
- Mahawithitwong, P. et al. (2013) Pancreas 42, 663-9.
- Shen, Z. et al. (2012) Am J Surg 203, 222-9.
- Yu, Y. et al. (2013) Int J Cancer 133, 1368-79.
- Gong, X. et al. (2010) Cancer Lett 299, 54-62.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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