R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
KIF5B (E4A5A) Rabbit mAb #62696
Filter:
- WB
- IP
- IF
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 110 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:200 - 1:800 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
KIF5B (E4A5A) Rabbit mAb recognizes endogenous levels of total KIF5B protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly408 of human KIF5B protein.
Background
Kinesin superfamily proteins (KIFs) are molecular motors that drive directional, microtubule-dependent intracellular transport of membrane-bound organelles and other macromolecules (e.g., proteins, nucleic acids). The intracellular transport functions of KIFs are fundamentally important for a variety of cellular functions, including mitotic and meiotic division, motility/migration, hormone and neurotransmitter release, and differentiation (1-4). Disruptions to KIF-mediated intracellular transport have been linked with a variety of pathologies, ranging from tumorigenesis to defects in higher order brain function such as learning and memory (4-6).
KIF5 consists of three family members referred to as KIF5A, KIF5B, and KIF5C (7). KIF5A and KIF5C are specifically expressed in neurons whereas KIF5B is expressed ubiquitously (7-10). Targeted disruption of the kif5B gene resulted in embryonic lethality (11). These and other studies demonstrate that KIF5B plays an important role in the localization and distribution of major organelles, including the mitochondria and lysosome, and can contribute to autophagy (11-13). In addition, gene rearrangements involving KIF5B fusions to ALK and RET have been identified as drivers for lung cancer and other malignancies (14-18).
KIF5 consists of three family members referred to as KIF5A, KIF5B, and KIF5C (7). KIF5A and KIF5C are specifically expressed in neurons whereas KIF5B is expressed ubiquitously (7-10). Targeted disruption of the kif5B gene resulted in embryonic lethality (11). These and other studies demonstrate that KIF5B plays an important role in the localization and distribution of major organelles, including the mitochondria and lysosome, and can contribute to autophagy (11-13). In addition, gene rearrangements involving KIF5B fusions to ALK and RET have been identified as drivers for lung cancer and other malignancies (14-18).
- Hirokawa, N. et al. (2009) Nat Rev Mol Cell Biol 10, 682-96.
- Yu, Y. and Feng, Y.M. (2010) Cancer 116, 5150-60.
- Park, J.J. et al. (2008) Mol Endocrinol 22, 989-1005.
- Hirokawa, N. et al. (2010) Neuron 68, 610-38.
- Yoshimura, Y. et al. (2010) Mol Cell Biol 30, 2206-19.
- Hirokawa, N. and Noda, Y. (2008) Physiol Rev 88, 1089-118.
- Nakagawa, T. et al. (1997) Proc Natl Acad Sci U S A 94, 9654-9.
- Aizawa, H. et al. (1992) J Cell Biol 119, 1287-96.
- Kanai, Y. et al. (2000) J Neurosci 20, 6374-84.
- Meng, Y.X. et al. (1997) Endocrinology 138, 1979-87.
- Tanaka, Y. et al. (1998) Cell 93, 1147-58.
- Cardoso, C.M. et al. (2009) PLoS One 4, e4424.
- Du, W. et al. (2016) Dev Cell 37, 326-336.
- Takeuchi, K. et al. (2009) Clin Cancer Res 15, 3143-9.
- Wong, D.W. et al. (2011) Cancer 117, 2709-18.
- Takeuchi, K. et al. (2012) Nat Med 18, 378-81.
- Ju, Y.S. et al. (2012) Genome Res 22, 436-45.
- Kohno, T. et al. (2012) Nat Med 18, 375-7.
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our
Trademark Information page.