R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
KIF5A (F2W1W) Rabbit mAb #92872
Filter:
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 125 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
KIF5A (F2W1W) Rabbit mAb recognizes endogenous levels of total KIF5A protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro817 of human KIF5A protein.
Background
Kinesin superfamily proteins (KIFs) are molecular motors that drive directional, microtubule-dependent intracellular transport of membrane-bound organelles and other macromolecules (e.g., proteins, nucleic acids). The intracellular transport functions of KIFs are fundamentally important for various cellular functions, including mitotic and meiotic division, motility/migration, hormone and neurotransmitter release, and differentiation (1-4). Disruptions to KIF-mediated intracellular transport have been linked with various pathologies, ranging from tumorigenesis to defects in higher-order brain functions such as learning and memory (4-6).
KIF5 consists of three family members: KIF5A, KIF5B, and KIF5C (7). KIF5A and KIF5C are specifically expressed in neurons, whereas KIF5B is expressed ubiquitously (7-10). KIF5A has recently also been found to be expressed in astrocyte processes and mutations of the protein have been linked to amyotrophic lateral sclerosis (ALS) (11-13).
KIF5 consists of three family members: KIF5A, KIF5B, and KIF5C (7). KIF5A and KIF5C are specifically expressed in neurons, whereas KIF5B is expressed ubiquitously (7-10). KIF5A has recently also been found to be expressed in astrocyte processes and mutations of the protein have been linked to amyotrophic lateral sclerosis (ALS) (11-13).
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- Meng, Y.X. et al. (1997) Endocrinology 138, 1979-87.
- Brenner, D. et al. (2018) Brain 141, 688-697.
- Szebényi, K. et al. (2023) Commun Biol 6, 678.
- Baron, D.M. et al. (2022) Cell Rep 39, 110598.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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