KCC2 Antibody #55473
Inquiry Info. # 55473
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Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 130-180 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
KCC2 Antibody recognizes endogenous levels of total KCC2 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln954 of human KCC2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The potassium/chloride cotransporter 2 (KCC2, SLC12A5) is a neuron-specific transport protein responsible for regulating the cotransport of potassium and chloride ions. KCC2 uses the energy of the electrochemical potassium gradient to export chloride ions from cells, therefore maintaining intracellular chloride ion concentrations in mature neurons (1,2). The intracellular concentration of chloride ions determines the neuronal response to the inhibitory neurotransmitter GABA and glycine. As a result, KCC2 can play a critical role in regulating neuronal excitability in mature central nervous system neurons (3-5). Altered KCC2 expression and reduced KCC2 activity can result in an increase in intracellular chloride ion concentrations and subsequent hyperexcitability of neuronal systems. Cases of aberrant KCC2 function are associated with neurological disorders, such as multiple forms of epilepsy, neuropathic pain, and schizophrenia (6-10).
- Payne, J.A. et al. (1996) J Biol Chem 271, 16245-52.
- Delpire, E. (2000) News Physiol Sci 15, 309-12.
- Rivera, C. et al. (1999) Nature 397, 251-5.
- Woo, N.S. et al. (2002) Hippocampus 12, 258-68.
- Zhu, L. et al. (2005) J Neurophysiol 93, 1557-68.
- Arion, D. and Lewis, D.A. (2011) Arch Gen Psychiatry 68, 21-31.
- Hyde, T.M. et al. (2011) J Neurosci 31, 11088-95.
- Kaila, K. et al. (2014) Curr Opin Neurobiol 26, 34-41.
- Mòdol, L. et al. (2014) Pain 155, 1577-90.
- Kahle, K.T. et al. (2014) EMBO Rep 15, 766-74.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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