R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
JMJD7 (E8J8Q) Rabbit mAb #85449
Filter:
- WB
Supporting Data
| REACTIVITY | H R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 35, 40 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
JMJD7 (E8J8Q) Rabbit mAb recognizes endogenous levels of total JMJD7 protein.
Species Reactivity:
Human, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro251 of human JMJD7 protein.
Background
The methylation state of lysine residues in histone proteins is a major determinant of the formation of active and inactive regions of the genome and is crucial for proper programming of the genome during development (1,2). Jumonji C (JmjC) domain-containing proteins represent the largest class of potential histone demethylase proteins (3). The JmjC domain can catalyze the demethylation of mono-, di-, and tri-methyl lysine residues via an oxidative reaction that requires iron and α-ketoglutarate (3). Based on homology, both humans and mice contain at least 30 such proteins, which can be divided into 7 separate families (3). JMJD7 is a member of the less studied JmjC family of oxygenates that catalyze formation of alcohol products. JMJD7 uniquely catalyzes (3S)-lysyl hydroxylation in members of the Translation Factor GTPase family (4). JMJD7, along with JMJD5, have also been found to function as proteases capable of cleaving histone tails containing methylated arginine (5,6). The JMJD7 gene can fuse with neighboring gene PLA2G4B to drive head and neck squamous cell carcinomas (7).
- Kubicek, S. et al. (2006) Ernst Schering Res Found Workshop, 1-27.
- Lin, W. and Dent, S.Y. (2006) Curr Opin Genet Dev 16, 137-42.
- Klose, R.J. et al. (2006) Nat Rev Genet 7, 715-27.
- Markolovic, S. et al. (2018) Nat Chem Biol 14, 688-695.
- Liu, H. et al. (2017) Proc Natl Acad Sci U S A 114, E7717-E7726.
- Liu, H. et al. (2018) Sci Rep 8, 3275.
- Cheng, Y. et al. (2017) Oncotarget 8, 1972-1982.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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