R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
IRF-9 (D8G7H) Rabbit mAb #28492
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 48 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
IRF-9 (D8G7H) Rabbit mAb recognizes endogenous levels of total IRF-9 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro362 of human IRF-9 protein.
Background
Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, IRF-9/ISGF3γ, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2).
In response to type I interferons (e.g., IFN-α, IFN-β), interferon regulatory factor 9 (IRF-9, ISGF3γ) assembles into a protein complex with Stat1 and Stat2 and translocates to the nucleus where it induces transcription of interferon response genes by binding to ISREs (3,4). Expression of IRF-9 is also induced by type I interferons (5).
In response to type I interferons (e.g., IFN-α, IFN-β), interferon regulatory factor 9 (IRF-9, ISGF3γ) assembles into a protein complex with Stat1 and Stat2 and translocates to the nucleus where it induces transcription of interferon response genes by binding to ISREs (3,4). Expression of IRF-9 is also induced by type I interferons (5).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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