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iNOS (E3W6B) Rabbit mAb #20609

Filter:
  • WB
  • IP
  • IF
Western blot analysis of extracts from DLD-1 cells, untreated (-) or treated with Human Interferon-γ (hIFN-γ) #80385 (100 U/mL), Human Interleukin-1β (hIL-1β) #8900 (50 U/mL), and Human Tumor Necrosis Factor-α (hTNF-α) #8902 (10 ng/mL) for 24 hr (+), using iNOS (E3W6B) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 20609

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 130
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50
Immunofluorescence (Immunocytochemistry) 1:800 - 1:1600

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

iNOS (E3W6B) Rabbit mAb recognizes endogenous levels of total iNOS protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human iNOS protein.

Background

Nitric Oxide Synthase (NOS) catalyzes the formation of nitric oxide (NO) and citrulline from L-arginine, oxygen, and cofactors. Three family members have been characterized: neuronal NOS (nNOS), which is found primarily in neuronal tissue; inducible NOS (iNOS), which is induced by interferon gamma and lipopolysaccharides in the kidney and cardiovascular system; and endothelial NOS (eNOS), which is expressed in blood vessels (1). NO is a messenger molecule with diverse functions throughout the body, including the maintenance of vascular integrity, homeostasis, synaptic plasticity, long-term potentiation, learning, and memory (2,3).

NO catalyzed by iNOS is involved in host defense against protozoa, bacteria, fungi, and viruses. Unlike constitutively expressed eNOS and nNos, iNOS is not usually expressed in quiescent cells. iNOS is transcriptionally induced in response to bacterial endotoxins, such as LPS and proinflammatory cytokines, in macrophages and various other cell types. Transcription factors involved in iNOS transcription include NF-κB, AP-1, and STAT. Different signaling pathways either promote (Jak1/2, PKC, c-Raf, p38 MAP kinase, and p44/42 MAP kinase) or inhibit (PI3 kinase) iNOS expression depending on stimulus and cell type (4).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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