R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
INF2 (E7H5D) Rabbit mAb #41081
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 170 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
INF2 (E7H5D) Rabbit mAb recognizes endogenous levels of total INF2 protein. INF2 isoforms 1 and 2 appear as a doublet in the 170 kDa range using western blot analysis. This antibody also detects a band at approximately 100 kDa of unknown origin. This band may be a carboxy-terminal cleavage product of INF2.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1019 of human INF2 protein.
Background
Inverted formin 2 (INF2) is a member of the formin family of actin assembly factors. INF2 regulates multiple cellular processes, including mitochondrial fission and vesicle trafficking. INF2 also regulates microtubule stability in conjunction with the formin mDia1 and IQGAP1 (1).
Mutations in INF2 have been linked to kidney disease and neuropathy in humans (2). INF2 stimulates Drp1-mediated and Drp1-independent mitochondrial division (3). Speckle-type POZ Protein (SPOP), a potential driver in human prostate cancer, regulates INF2 activity through ubiquitination, affecting INF2-mediated migration, invasion, and mitochondrial fission in prostate cancer (4). In thyroid cancer, JNK2 signaling mediates INF2-related mitochondrial fission to promote apoptosis (5).
Mutations in INF2 have been linked to kidney disease and neuropathy in humans (2). INF2 stimulates Drp1-mediated and Drp1-independent mitochondrial division (3). Speckle-type POZ Protein (SPOP), a potential driver in human prostate cancer, regulates INF2 activity through ubiquitination, affecting INF2-mediated migration, invasion, and mitochondrial fission in prostate cancer (4). In thyroid cancer, JNK2 signaling mediates INF2-related mitochondrial fission to promote apoptosis (5).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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