IMPDH1 Antibody #28420
Filter:
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 55, 60 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
IMPDH1 Antibody recognizes endogenous levels of total IMPDH1 protein. This antibody does not cross-react with IMPDH2 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val130 of human IMPDH1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Inosine-5'-monophosphate dehydrogenase (IMPDH) catalyzes the conversion of IMP to XMP, the first and rate-limiting step of guanine nucleotide biosynthesis (1). The isozymes IMPDH1 and IMPDH2 are encoded by two distinct genes (2). Research studies show that IMPDH2 is overexpressed in glioblastoma. In glioblastoma, IMPDH2-mediated GTP biosynthesis is essential for rRNA and tRNA synthesis and the formation of enlarged and irregular nucleoli. In addition, IMPDH1 and IMPDH2 double knockout leads to significant inhibition of tumor growth in an orthotopic glioblastoma model (3). Furthermore, IMPDH1 and IMPDH2 expression is upregulated in small cell lung cancer cells with low expression of ASCL1 transcription factor (ASCL1Low). Suppression of IMPDH activity inhibits ASCL1Low small cell lung cancer growth (4).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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