R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
ILF3 (E5H6F) Rabbit mAb #39249
Filter:
- WB
- IHC
- IF
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 85, 110 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:1000 - 1:4000 |
| Immunofluorescence (Immunocytochemistry) | 1:200 - 1:400 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
ILF3 (E5H6F) Rabbit mAb recognizes endogenous levels of total ILF3 protein. Species reactivity for IHC-P is human only.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val353 of human ILF3 protein.
Background
ILF2 and ILF3, also known as NF45 and NF90 respectively, form a double-stranded RNA binding heterodimer that serves many different biological functions. ILF2 and ILF3 were originally discovered as proteins that can bind and regulate the antigen receptor response element of the IL2 promoter to contribute to T cell activation (1,2). ILF2 and ILF3 play important roles in DNA damage response and non-homologous end joining (NHEJ) by interacting with DNA-PK (3,4). The heterodimer can also bind and negatively regulate microRNAs (5,6). Biogenesis of the 60S ribosomal subunit has been shown to be dependent on the double-stranded RNA-binding domains of ILF3 (7). Additionally, ILF2 and ILF3 can mediate rDNA transcription (8). ILF2 and ILF3 also play a role in the life cycle of many different viruses, including dengue and hepatitis C (9-11).
- Flanagan, W.M. et al. (1991) Nature 352, 803-7.
- Kao, P.N. et al. (1994) J Biol Chem 269, 20691-9.
- Ting, N.S. et al. (1998) J Biol Chem 273, 2136-45.
- Shamanna, R.A. et al. (2011) Mol Cell Biol 31, 4832-43.
- Sakamoto, S. et al. (2009) Mol Cell Biol 29, 3754-69.
- Masuda, K. et al. (2013) Int J Mol Sci 14, 17111-21.
- Wandrey, F. et al. (2015) Mol Cell Biol 35, 3491-503.
- Tsai, H.I. et al. (2021) EMBO Mol Med 13, e12834.
- Isken, O. et al. (2003) EMBO J 22, 5655-65.
- Gomila, R.C. et al. (2011) PLoS One 6, e16687.
- Isken, O. et al. (2007) RNA 13, 1675-92.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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