R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
IL4R (F6C4Z) Rabbit mAb #56538
Filter:
- WB
- IP
- F
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 75-80, 120 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- F-Flow Cytometry
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Flow Cytometry (Fixed/Permeabilized) | 1:100 - 1:400 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
IL4R (F6C4Z) Rabbit mAb recognizes endogenous levels of total IL4R protein.
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse IL4R protein.
Background
In the immune system, interleukin-4 (IL-4) controls B cell growth, survival, and maturation, in addition to proliferation and differentiation of the Th2 subset of T cells. IL-4 is a cytokine secreted by activated T cells, basophils, and mast cells (1,2). It acts through the IL-4 receptor (IL4R), leading to tyrosine phosphorylation and activation of the Stat6 transcription factor (3).
IL4R is a transmembrane ligand binding protein composed of a heterodimer alpha and gamma receptor complex (4,5). IL4R has been shown to bind both IL-4 and interleukin-13 (IL-13), regulating immunoglobulin E (IgE) and chemokine production during allergic inflammation (6). In addition to its role in regulating immune response, signaling through IL4R is also implicated in diseases such as asthma, allergy, and cancer, making it a potent target for various therapies (7-9). Increased IL4R expression has been observed in tumors (9,10), and several mutations in IL4R are associated with metastatic cancers, underscoring its importance in cancer progression and as a target for chemotherapy development (10,11).
IL4R is a transmembrane ligand binding protein composed of a heterodimer alpha and gamma receptor complex (4,5). IL4R has been shown to bind both IL-4 and interleukin-13 (IL-13), regulating immunoglobulin E (IgE) and chemokine production during allergic inflammation (6). In addition to its role in regulating immune response, signaling through IL4R is also implicated in diseases such as asthma, allergy, and cancer, making it a potent target for various therapies (7-9). Increased IL4R expression has been observed in tumors (9,10), and several mutations in IL4R are associated with metastatic cancers, underscoring its importance in cancer progression and as a target for chemotherapy development (10,11).
- Yokota, T. et al. (1986) Proc Natl Acad Sci U S A 83, 5894-8.
- Grabstein, K. et al. (1986) J Exp Med 163, 1405-14.
- Hou, J. et al. (1994) Science 265, 1701-6.
- Keegan, A.D. et al. (1994) Cell 76, 811-20.
- Naz, S. et al. (2019) Int J Biol Macromol 123, 239-245.
- Reece, P. et al. (2014) PLoS One 9, e100734.
- Massoud, A.H. et al. (2016) Nat Med 22, 1013-22.
- Aranda, C.J. et al. (2023) Allergy 78, 752-766.
- Viganò, E. et al. (2018) Blood 131, 2036-2046.
- Bankaitis, K.V. and Fingleton, B. (2015) Clin Exp Metastasis 32, 847-56.
- de Groot, A.E. et al. (2022) Neoplasia 32, 100830.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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