R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
IL3RA/CD123 (E8B3N) Rabbit mAb #59343
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 70 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
IL3RA/CD123 (E8B3N) Rabbit mAb recognizes endogenous levels of total IL3RA/CD123 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human IL3RA/CD123 protein.
Background
IL3RA/CD123 is the alpha subunit of an IL-3 receptor that also contains the common beta chain (βc) shared with receptors for other cytokines. IL3RA/CD123 alone binds to IL-3 with high specificity but low affinity and it does not transduce intracellular signaling, while βc alone does not bind to IL-3 appreciably. Together, IL3RA/CD123 and βc form the functional high-affinity receptor for IL-3. IL3RA/CD123 is mainly expressed on the hematopoietic progenitor cells, primarily the granulocytic and monocytic linages (1,2). IL3RA/CD123 is highly expressed on leukemic stem cells in the majority of AML (acute myeloid leukemia) patients and is correlated with poor prognosis. In addition, overexpression of IL3RA/CD123 has also been reported for other hematopoietic malignancies (3-5). It is considered as a valuable therapeutic target for these malignancies (6-8).
- Miyajima, A. (1992) Int J Cell Cloning 10, 126-34.
- Goodall, G.J. et al. (1993) Growth Factors 8, 87-97.
- Muñoz, L. et al. (2001) Haematologica 86, 1261-9.
- Testa, U. et al. (2002) Blood 100, 2980-8.
- Testa, U. et al. (2004) Leukemia 18, 219-26.
- Liu, K. et al. (2015) Life Sci 122, 59-64.
- Pemmaraju, N. (2017) Curr Hematol Malig Rep 12, 510-12.
- Cummins, K.D. and Gill, S. (2018) Leuk Lymphoma 59, 1539-53.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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