R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
IL1RAP (E6W2S) Rabbit mAb #52686
Filter:
- WB
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 78 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
IL1RAP (E6W2S) Rabbit mAb recognizes endogenous levels of total IL1RAP protein.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human IL1RAP protein.
Background
Interleukin-1 receptor accessory protein (IL1RAP/IL1R3) is a type-I transmembrane glycoprotein and component of the IL-1 and IL-33 receptor signaling complexes (1,2). Research studies have demonstrated that IL1RAP expression is required for IL-1-mediated signaling through its ability to recruit IRAK to the IL-1R complex (3-7). In the context of human disease, research studies have shown that IL1RAP is overexpressed on the surface of AML stem cells and associates with c-KIT and FLT3 to mediate pro-proliferative and pro-survival signaling events downstream of these oncogenic receptor tyrosine kinases (8,9). As such, IL1RAP has emerged as a novel immuno-therapeutic target for AML and other malignancies of the myeloid compartment (10,11).
- Greenfeder, S.A. et al. (1995) J Biol Chem 270, 13757-65.
- Chackerian, A.A. et al. (2007) J Immunol 179, 2551-5.
- Radons, J. et al. (2002) J Biol Chem 277, 16456-63.
- Huang, J. et al. (1997) Proc Natl Acad Sci U S A 94, 12829-32.
- Wesche, H. et al. (1997) J Biol Chem 272, 7727-31.
- Gould, L. et al. (1979) J Clin Pharmacol 19, 46-55.
- Volpe, F. et al. (1997) FEBS Lett 419, 41-4.
- Mitchell, K. et al. (2018) J Exp Med 215, 1709-27.
- Barreyro, L. et al. (2012) Blood 120, 1290-8.
- Askmyr, M. et al. (2013) Blood 121, 3709-13.
- Ågerstam, H. et al. (2015) Proc Natl Acad Sci U S A 112, 10786-91.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
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