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IL-6 (Siltuximab Biosimilar) Human mAb #52897
Binding kinetics of IL-6 (Siltuximab Biosimilar) Human mAb to recombinant IL-6 were measured using bio-layer interferometry (BLI) on the Octet RED96 (Sartorius). Antibodies were immobilized to ProA biosensors (Sartorius), which were then dipped into wells containing a 2-fold dilution series of recombinant IL-6. Concentrations tested ranged from 1,000 nM to 15.6 nM. Global curve-fitting was performed using a 1:1 binding model; only the concentrations that passed that fitting are shown.
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Human IgG1 kappa |
Species Cross-Reactivity Key:
- H-Human
- Related Products
Product Information
Product Description
IL-6 (Siltuximab Biosimilar) Human mAb is a biosimilar antibody for Siltuximab. Siltuximab is an FDA-approved human monoclonal antibody directed against IL-6. Siltuximab may help reduce inflammation and stop the growth of cancer cells or abnormal blood cells.
| Endotoxin | <0.1 EU/μg of antibody |
Product Usage Information
This product is intended for research use only (RUO). Optimal dilutions/concentrations should be determined by the end user.
Formulation
Supplied in 1X PBS, BSA and Azide Free.
Storage
Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Specificity / Sensitivity
IL-6 (Siltuximab Biosimilar) Human mAb was confirmed to bind to its intended target protein IL-6 using bio-layer interferometry.
Species Reactivity:
Human
Source / Purification
IL-6 (Siltuximab Biosimilar) Human mAb is produced at Cell Signaling Technology. Siltuximabb is an antibody directed against human IL-6.
Background
Interleukin-6 (IL-6) is a pleiotropic cytokine with central roles in immune regulation, inflammation, hematopoiesis, and oncogenesis. Its biological activities are shared by other IL-6 family members, such as leukemia inhibitory factor and oncostatin M (1). To exert its biological effects, IL-6 must bind to the IL-6 receptor (IL-6R), composed of two alpha chains (IL-6Rα, 80 kDa) and two beta chains (IL-6Rβ or gp130, 130 kDa). Two moieties of IL-6 and two pairs of these receptor chains form a functional hexameric IL-6R complex. The subsequent intracellular signaling events are activated via gp130, which is the common signal transducer of cytokines related to IL-6. Activation of the IL-6R stimulates at least three major signaling pathways: JAK/STAT, PI-3 kinase, and MAPK signaling pathways (2,3).
Pathways
Explore pathways related to this product.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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