R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
IL-4 (D19A10) Rabbit mAb #12227
Filter:
- WB
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 17 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Flow Cytometry (Fixed/Permeabilized) | 1:800 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
IL-4 (D19A10) Rabbit mAb recognizes endogenous levels of total IL-4 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human IL-4 protein.
Background
Interleukin-4 (IL-4) is a cytokine secreted by activated T cells, basophils, and mast cells (1,2). While it contributes to many immunomodulatory responses, it is mainly recognized as the cytokine responsible for eliciting differentiation of naive T cells into Th2 lineage cells that are defined by their secretion of IL-4, IL-5, and IL-10 (3). In addition, IL-4 contributes to immunoglobulin class switching by inducing the production of IgE from B cells (4,5). IL-4 acts through the IL-4 receptor, leading to tyrosine phosphorylation and activation of the Stat6 transcription factor (6).
- Yokota, T. et al. (1986) Proc Natl Acad Sci USA 83, 5894-8.
- Grabstein, K. et al. (1986) J Exp Med 163, 1405-14.
- Kopf, M. et al. (1993) Nature 362, 245-8.
- Kotowicz, K. and Callard, R.E. (1993) Eur J Immunol 23, 2250-6.
- Thyphronitis, G. et al. (1989) Proc Natl Acad Sci USA 86, 5580-4.
- Hou, J. et al. (1994) Science 265, 1701-6.
限制使用
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