IGFBP3 Antibody #13216
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Product Name
Filter:
- WB
Western blot analysis of concentrated conditioned media from serum-starved A-431 cells, untreated (-) or treated with Human Insulin-like Growth Factor I (hIGF-I) #8917 (100 nM, 12 hr; +), using IGFBP3 Antibody.
This product is discontinued
Inquiry Info. # 13216
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Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 40 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
IGFBP3 Antibody recognizes endogenous levels of total IGFBP3 protein.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser155 of human IGFBP3 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Insulin-like growth factor (IGF) signaling plays a major role in regulating the proliferation and metabolism of normal and malignant cells. Insulin-like growth factor-binding proteins (IGFBPs) play an integral role in modifying IGF actions in a wide variety of cell types. The six IGFBP family members share a high affinity for IGF binding and are structurally related, but are encoded by distinct genes (1). IGFBPs can exert stimulatory or inhibitory effects by controlling IGF availability through high affinity binding of IGF at the carboxy-terminal domain (2,3). IGFBP3 is the most abundant serum IGFBP and the main mediator for IGF-I bioactivities. IGFBP3 also binds IGF-II, insulin, and other cellular and extracellular components to regulate cell growth, development, and apoptosis through both IGF-dependent and IGF-independent mechanisms (4-8). Research studies describe correlations between increased IGF-I levels and reduced levels of IGFBP3 with increased risks of developing cancer, including breast, colon, lung, and prostate cancer (2).
- Hwa, V. et al. (1999) Endocr Rev 20, 761-87.
- Yu, H. and Rohan, T. (2000) J Natl Cancer Inst 92, 1472-89.
- Martin, J.L. and Baxter, R.C. (2011) Growth Factors 29, 235-44.
- Zapf, J. et al. (1990) J Biol Chem 265, 14892-8.
- Coverley, J.A. and Baxter, R.C. (1997) Mol Cell Endocrinol 128, 1-5.
- Ingermann, A.R. et al. (2010) J Biol Chem 285, 30233-46.
- Liu, B. et al. (2000) J Biol Chem 275, 33607-13.
- Baxter, R.C. (2001) Mol Pathol 54, 145-8.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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