R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
IGF-II Receptor/CI-M6PR (D8Z3J) Rabbit mAb #15128
Filter:
- WB
- IHC
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 275 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
IGF-II Receptor/CI-M6PR (D8Z3J) Rabbit mAb recognizes endogenous levels of total IGF-II receptor/CI-M6PR protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe1379 of human IGF-II receptor/CI-M6PR protein.
Background
Insulin-like growth factor II (IGF-II) receptor, also widely known as cation-independent mannose 6-phosphate receptor (CI-M6PR), is a multifunctional type I transmembrane glycoprotein that participates in the internalization of mannose-6-phosphate modified hydrolases and IGF-II from the plasma membrane (1,2). In the absence of ligands, IGF-II receptor is constitutively endocytosed from the cell surface to accumulate in the Golgi apparatus (3). In the presence of ligands, the receptor transports the mannose-6-phosphate modified hydrolases to acidified endosomes and lysosomes (4). The ligand-free receptor is then transported back to the Golgi compartment or the cell surface (4). In several research studies, IGF-II receptor has been recognized as a tumor suppressor in a number of cancers (5-7).
- Lobel, P. et al. (1989) Cell 57, 787-96.
- Kiess, W. et al. (1988) J Biol Chem 263, 9339-44.
- York, S.J. et al. (1999) J Biol Chem 274, 1164-71.
- Duncan, J.R. and Kornfeld, S. (1988) J Cell Biol 106, 617-28.
- Oates, A.J. et al. (1998) Breast Cancer Res Treat 47, 269-81.
- Martin-Kleiner, I. and Gall Troselj, K. (2010) Cancer Lett 289, 11-22.
- Puxbaum, V. et al. (2012) J Hepatol 57, 337-43.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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