IDH2 (KrMab-3) Mouse mAb #60322
Filter:
- WB
- IP
- IHC
- IF
- F
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 43 |
| Source/Isotype | Mouse IgG2b |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
| Immunofluorescence (Immunocytochemistry) | 1:50 |
| Flow Cytometry (Fixed/Permeabilized) | 1:50 - 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
IDH2 (KrMab-3) Mouse mAb recognizes endogenous levels of total IDH2 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala174 of human IDH2 protein, as described by Kaneko et al. (2013) [8].
Background
IDH2 is one of three isocitrate dehydrogenases (IDH1-3) that catalyze the oxidative decarboxylation of isocitrate to produce CO2 and α-ketoglutarate (α-KG). These enzymes belong to two distinct subclasses that utilize either NAD or NADP+ as an electron acceptor. IDH2 is an NADP+-dependent isocitrate dehydrogenase expressed primarily in the mitochondria, where it also functions in the TCA cycle (1,2). Mutations in IDH2 or its cytoplasmic counterpart (IDH1) have been reported in glioblastoma multiforme (3), acute myeloid leukemia (4,5), and other malignancies (6). Research studies have shown that gain-of-function mutations in IDH2 can lead to the accumulation and secretion of the oncometabolite R-2-hydroxyglutarate (2HG) in cancer cells (6,7).
- Wise, D.R. et al. (2011) Proc Natl Acad Sci U S A 108, 19611-6.
- Filipp, F.V. et al. (2012) Pigment Cell Melanoma Res 25, 375-83.
- Parsons, D.W. et al. (2008) Science 321, 1807-12.
- Abbas, S. et al. (2010) Blood 116, 2122-6.
- Paschka, P. et al. (2010) J Clin Oncol 28, 3636-43.
- Watanabe, T. et al. (2009) Am J Pathol 174, 1149-53.
- Pardanani, A. et al. (2010) Leukemia 24, 1370-2.
- Kaneko, M.K. et al. (2013) Biochem Biophys Res Commun 432, 40-5.
限制使用
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