R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
ID1 (F2M1J) Rabbit mAb (IF/Flow Formulated) #25344
Filter:
- IF
- F
Confocal immunofluorescent analysis of HCT 116 cells (left, positive), KYSE-150 cells (middle, low-expressing), and NCI-H929 cells (right, negative) using ID1 (F2M1J) Rabbit mAb (IF/Flow Formulated) (green), Cytochrome c (6H2.B4) Mouse mAb #12963 (red), and DAPI #4083 (blue).
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Immunocytochemistry) | 1:1000 |
| Flow Cytometry (Fixed/Permeabilized) | 1:50 - 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
ID1 (F2M1J) Rabbit mAb (IF/Flow Formulated) recognizes endogenous levels of total ID1 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human ID1 protein.
Background
Inhibitor of DNA-binding/Differentiation (ID) proteins are a family of proteins that function to repress the activity of basic helix-loop-helix (bHLH) transcription factors. There are four known ID proteins in humans (ID1-4), all of which contain a helix-loop-helix domain but lack a basic DNA-binding domain. Heterodimerization with bHLH transcription factors, therefore, functions to sequester bHLH proteins and prevent their binding to DNA (1). ID proteins play important functional roles in development, primarily by inhibiting premature differentiation of stem/progenitor cells (1,2). ID1 plays important regulatory roles downstream of TGF-β and BMP signaling by inhibiting various transcription factors (3,4). SMAD3 is capable of binding to the ID1 promoter, causing upregulation in response to TGF-β (5). High expression of ID1 is associated with increased cancer growth and migration by inhibiting cell cycle regulators p16, p21, and p27 (6,7). ID1 as a therapeutic target is context dependent. While high expression of ID1 is generally associated with poor prognosis, drug sensitivity can vary greatly depending on tumor type (8-10).
- Yokota, Y. (2001) Oncogene 20, 8290-8.
- Hong, S.H. et al. (2011) J Cell Sci 124, 1445-52.
- Di, K. et al. (2006) Biol Cell 98, 523-33.
- Miyazono, K. and Miyazawa, K. (2002) Sci STKE 2002, pe40.
- Liang, Y.Y. et al. (2009) Cell Res 19, 140-8.
- Asirvatham, A.J. et al. (2007) Prostate 67, 1411-20.
- Alani, R.M. et al. (2001) Proc Natl Acad Sci USA 98, 7812-6.
- Castañon, E. et al. (2013) J Transl Med 11, 13.
- Li, L. et al. (2017) Oncol Lett 13, 3014-3024.
- Tan, H.Y. et al. (2020) Cancer Lett 475, 109-118.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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